EFFECT OF VITAMIN A DEFICIENCY ON KIDNEY OXIDATIVE STRESS AND INFLAMMATION

BIAGGIO VS, ALVAREZ SM, GATICA LV, GOMEZ NN, OLIVEROS LB, GIMENEZ SM.

Mendoza

Otro; SOCIEDAD DE BIOLOGIA DE CUYO - XX REUNION CIENTIFICA ANUAL; 2002

Universidad Nacional de Cuyo

Abstract Vitamin A participates in tissue differentiation, growth and maintenance. We have showed that vitamin A deficiency modifies kidneys lipidic profile and increases superoxide dismutase (SOD) activity. We studied the effect of this deficiency on iNOS and COX- 2 expression in renal papilla and medulla. Male Wistar rats, 21- day old, were fed during three months with vitamin A-free diet (- A) and the same diet plus 8 mg of retinol palmitate/kg of diet (+A). Renal papillae and medullae were homogenized in presence of protease inhibitors and proteins were measured. 40 mg of proteins were loaded on 8% SDS-PAGE gel. iNOS and COX-2 were identified by Western blot with specific antibodies and bands were revealed using Vectastain ABC detection kit. Both tissues (papilla and medulla) showed iNOS and COX-2 expression without significant differences between –A and +A. Then even if there is some oxidative stress, it does not modify the expression of these enzymes.