EFFECT OF CADMIUM BIOTOXICITY AND SOIL PROTEIN DURING RAT GESTATION

BIAGGIO VS; ALTAMIRANO KN; GIMENEZ MS

Buenos Aires

Congreso; Sociedad Argentina de Investigación Clínica; 2017

Sociedad Argentina de Investigación Clínica

Introduction: the placenta is the main known tissue to protect the fetus from environmental toxins, such as cadmium (Cd). This divalent ion is a cytotoxic endocrine disruptor and produces an imbalance in the transport of nutrients through the placenta, thus causing a decrease in fetal birth weight or premature births. Several studies show that at very low doses, Cd can induce fetal growth restriction, thus increasing the risk of neonatal morbidity and mortality or generating in adult life diseases such as diabetes, obesity or coronary heart disease. Soy protein is becoming increasingly important in the human diet. Santell et al. (1997) demonstrated that isoflavones (genistein) could cause hypertrophy in the rat endometrium and alter reproductive function in numerous species. Objective: to evaluate the possible protective role of the consumption of soy protein compared to the mechanisms by which Cd exerts its toxicity. Materials and Methods: 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water during pregnancy period (20G). Results: We determined TBARS, catalase (CAT) and glutation peroxidase (GPx) activity, and nitrite concentration. Placenta tissue total RNA was extracted and RT-PCR was performed using the following primers: MT I; MT II; Nrf-2; NOX-2, SOD-2 and CAT. The concentration of Cd increased in both intoxicated groups (p