EFECTOS DE LA CASTRACION Y SUSTITUCION CON TESTOSTERONA SOBRE LA EXPRESIÓN DEL MAYOR FACTOR DE TRANSCRIPCION ANTIOXIDANTE DEL PULMON

PÉREZ CHACA, MV; BIAGGIO VS; PIGUILLEM SN; NOLLAC V; GIMENEZ MS; GOMEZ NN

Otro; SOCIEDAD de BIOLOGIA DE CUYO (SOBIO); 2010

SOCIEDAD de BIOLOGIA DE CUYO (SOBIO)

Oxidative stress plays a key role of in several lung diseases and Nrf2, a major antioxidant transcription factor could play a protective role in pulmonary disease. The presence of androgen receptors in the lung implies that sex hormones play a physiological role in pulmonary function. The present study was designed to determine whether oxidative stress after castration and androgen replacement was modified by Nrf2 pathway.Wistar male rats (200 ± 20 g) were separated in three groups: controls (Co, n:6), castrated (Ca, n:6), and castrated replaced with testosterone (Ca+T, n:6) 60 days after castrations. RNA was extracted by the method of TRIzol. The level of RNAm was quantified using RT-PCR and oxidative stress biomarkers were measured. ANOVA was used for statistical analysis. The results indicate that castration significantly affected the antioxidant status, it is evidenced by a significant increase of TBAR?S (mM/mg protein) (p