¿QUIERES UN TRAGO? ESTRÉS OXIDATIVO DEL SISTEMA CARDIOVASCULAR PRODUCIDO POR LA INGESTA DE AGUA CONTAMINADA CON CADMIO

FERRAMOLA M; CORIA M; BIAGGIO VS; ANZULOVICH AC; GIMENEZ MS

Mar del Plata

Congreso; SOCIEDAD ARGENTINA DE INVESTIGACION BIOQUIMICA Y BIOLOGIA MOLECULAR (SAIB); 2007

SOCIEDAD ARGENTINA DE INVESTIGACION BIOQUIMICA Y BIOLOGIA MOLECULAR (SAIB)

Cadmium intoxication increases oxidative stress parameters in several tissues. Objectives: The aim of this study was to assess the cardiovascular effect of drinking cadmium contaminated water. Experimental model: Male Wistar rats were separated into four groups: 1 (Controls, tap water), 2, 3 and 4 (tap water with 15 ppm Cd ion, during 15, 30 and 60 days respectively). Food and water were administrated ad libitum. Afterwards, animals were decapitated, under light ether anesthesia. Samples were kept at - 20ºC, until processed. Methods: Determinations in serum: TBARS (Jentzsch ., 1969), Proteins (Layne ., 1957), NO (Schulkz et al., 1999), and Paraoxonase-1 (PON-1) (Beltowsky ., 2002). TBARS and iNOS expression (Western Blot) was assessed in heart. Results: Serum: TBARS were elevated in groups 3 and 4, (p