CADMIO MODIFICA LA SECRECION DE PROLACTINA POR DISTINTAS VIAS

CALDERONI AM; GATICA LV; BIAGGIO VS; GIMENEZ MS

Potrero de los Funes, San Luis

Encuentro; Sociedad de Biologia de Cuyo; 2007

Sociedad de Biologia de Cuyo

We have observed that exposition of adult male rats to 15 ppm of cadmium through the drinking water for two weeks, modified the prolactine (Pl) amount in pituitary by inmunohistochemitry determination and also decrease in serum. In order to determine the mechanism of cadmium action, we measured: the expression of mRNA of Pl by RT-PCR, the expression iNOS, TGF beta 1 and COX 2 by Western Blot using specific antibody. We observed that mRNA of Pl, and the amount of protein iNOS increase, while the amount of protein TGF beta 1 decrease and protein COX2 did not change. It is known that NO directly inhibits prolactin secretion and prolactine inhibit the expression of the inducible nitric oxide synthase (iNOS). On the other hand TGF-beta 1 suppressed PRL mRNA in a dose- and time-dependent manner,negatively controls the expression of iNOS and increase the expression of COX2. In consecuence we could say that cadmiun decrease the prolactine synthesis by different way: to postrancripcional level, and trough NO producction and down regulation of TGF beta 1.