DO YOU WANT A DRINK? CARDIOVASCULAR OXIDATIVE STRESS PRODUCED BY DRINKING Cd CONTAMINATEDWATER

FERRAMOLA ML, CORIA M, BIAGGIOV, ANZULOVICH AC, GIMÉNEZ MS.

Mar del Plata

Congreso; Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB); 2007

Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

Cadmium intoxication increases oxidative stress parameters in several tissues. Objectives: The aim of this study was to assess the cardiovascular effect of drinking cadmium contaminated water. Experimental model: Male Wistar rats were separated into tour groups: 1 (Controls, tap water), 2, 3 and 4 (tap water with 15 ppm Cd ion, during 15, 30 and 60 days respectively). Food and water were administrated ad libitum. Afterwards, animals were decapitated, under light ether anesthesia. Samples were kept at - 20ºC, until processed. Methods: Determinations in serum:TBARS (Jentzsch ., 1969), Proteins (Layne ., 1957), NO (Schulkz., 1999), and Paraoxonase-1 (PON-1) (Beltowsky ., 2002). TBARS and iNOS expression (Western Blot) was assessed in heart. Results: Serum: TBARS were elevated in groups 3 and 4, (p<0.05). PON-1 activity in group 3 was the highest (p<0.001). PON-1 activity augmented in group 4 compared to controls (p<0.001). NO determination showed no differences among the groups. Heart:TBARSincreased in group 4, (p<0.05). It was seen a decrease in iNOS production in groups 2 and 3. Conclusions: Rats exposure to oral cadmium intoxication increases oxidative stress parameters in serum and heart. It also seems to be an induction of antioxidant enzymes (PON-1) in serum at the first stages of intoxication, with a decreased iNOS concentration in heart.