EFFECT OF CADMIUN IN THE DRINKING WATER ON THE EXPRESSION OF PROLACTIN GENE AND NAPH OXIDASEIN PITUITARYGLAND

CALDERONI AM, BIAGGIOV,GIMENEZ MS

Mar del Plata

Congreso; Sociedad Argentina de Bioquímica y Biología Molecular (SAIB); 2007

Sociedad Argentina de Bioquímica y Biología Molecular (SAIB)

Cadmium (Cd (2+)) is an ubiquitous toxic metal that is involved in a variety of pathological conditions. Our previous result have shown that Cadmium (Cd) expression modify the lipid metabolism of pituitary gland. On the other hand it is known that Cd(2+)-mediates cytotoxicity and the inhibition of prolactin release, supporting the involvement of oxidative stress in the mechanism of Cd(2+) action. NADP oxidase is a generator of species oxygen reactive. Our objective was determine if Cd modify the expression of NADP oxidase, as generator of oxidative stress. Also, the relation of NADP oxidase and prolactin gene expression, was studied.Wistar adult male rats were exposed to 15 ppm of Cd as Cl2Cd in the drinking water during 8 weeks. Then, rats were sacrified and pituitary gland was extracted. The mRNA was isolated by Trizol reagent and the expression of NADP oxidase and prolactin genes were determine by RT-PCR. We observed that Cd did not modify the expression of mRNA NAPH oxidase and decreased the expression of mRNA Prolactin. We have previously shown a decreased secretion of prolactin, in this experimental condition, which could be attributed to a decreased expression of mRNA prolactin independent of oxidative stress.