EFECTOS BIOQUÍMICOS Y MOLECULARES DE DIFERENTES PROTEÍNAS DIETARIAS SOBRE EL CEREBRO DE FETOS A LOS 20 DÍAS DE GESTACIÓN

BIAGGIO VS; ALTAMIRANO KN; GIMENEZ MS

Congreso; SOCIEDAD DE BIOLOGIA DE CUYO; 2014

BIOCHEMICAL AND MOLECULAR EFFECTS OF DIFFERENT DIETARY PROTEIN ON FETAL BRAIN TO 20 DAYS PREGNANCY Biaggio VS, Altamirano K, Gimenez MS. Universidad Nacional de San Luis ? IMIBIO ? CONICET. Email: vbiaggio@gmail.com Cadmium (Cd) is an environmental contaminant known to exert significant neurotoxic effects on both humans and experimental animals. Exposure to Cd during gestation and lactation (the critical periods of neurodevelopment) can induce changes in the offspring central nervous systems antioxidant defense mechanisms. To evaluate the possible protective role of the consumption of soy protein compared to the mechanisms by which Cd exerts its toxicity, 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water during pregnancy period. We determined TBARS, catalase (CAT) and glutation peroxidase (GPx) activity, and nitrite concentration. Fetal tissue total RNA was extracted and RT-PCR was performed using the following primers: MT I; MT II; Nrf-2; NOX-2 and SOD. The concentration of Cd increased in both intoxicated groups (p