INVESTIGADORES
BIAGGIO Veronica Silvina
congresos y reuniones científicas
Título:
EFECTOS DE LA CASTRACION Y SUSTITUCION CON TESTOSTERONA SOBRE LA EXPRESIÓN DEL MAYOR FACTOR DE TRANSCRIPCION ANTIOXIDANTE DEL PULMON
Autor/es:
PÉREZ CHACA, MV, BIAGGIO VS, PIQUILLEM, SN, NOLLAC, V, GIMÉNEZ MS, GÓMEZ NN
Lugar:
Mendoza- Ciudad
Reunión:
Congreso; SOCIEDAD de BIOLOGIA DE CUYO (SOBIO); 2010
Institución organizadora:
SOCIEDAD de BIOLOGIA DE CUYO (SOBIO)
Resumen:
Oxidative stress plays a key role of in several lung diseases and
Nrf2, a major antioxidant transcription factor could play a protective
role in pulmonary disease. The presence of androgen receptors
in the lung implies that sex hormones play a physiological role in
pulmonary function.
The present study was designed to determine whether oxidative
stress after castration and androgen replacement was modified by
Nrf2 pathway.Wistar male rats (200 ± 20 g) were separated in three
groups: controls (Co, n:6), castrated (Ca, n:6), and castrated replaced
with testosterone (Ca+T, n:6) 60 days after castrations. RNA
was extracted by the method of TRIzol. The level of RNAm was
quantified using RT-PCR and oxidative stress biomarkers were
measured. ANOVA was used for statistical analysis. The results
indicate that castration significantly affected the antioxidant status,
it is evidenced by a significant increase of TBARS (mM/mg
protein) (p