INFLAMMATION IN CEREBRAL CORTEX INDUCED BY CADMIUM IN WISTAR RATS

RUSSO, M; BIAGGIO, V; SANTILLAN, L; GIMENEZ, MS

Mendoza

Congreso; XXVI Reunion Anual de la Sociedad de Biologia de Cuyo; 2008

Sociedad de Biologia de Cuyo

It has been observed that cadmium causes changes in the level of expression of genes involved in inflammatory disorders of different types cell in cerebral cortex. In this work was studied in cerebral cortex, the effect of cadmium on the expression of inflammatory parameters, also measured the expression of iNOS and parameters of oxidative stress (TBARS and NO). Our experimental model consisted of adult-male Wistar rats subjected to 15 ppm of cadmium in drinking water (Cd group) and without this (group CO). The total RNA was extracted by the method of TRIzol and levels of RNAm quantified using RT-PCR, the TBARS were quantified according, and the levels of NO using the reaction of Gries, Schulz et al., 1999. We observed a significant increase in TNF(CO: 0.795±0.034; Cd: 0.899±0.053 p: 0.0168) and VCAM- 1 (CO: 0.302±0.106; Cd: 0.56±0.056; p: 0.0053), but there was no significant difference of PPAR(CO: 0.495±0.069; Cd: 0.561±0.06; p: 0.207); the TBARS increased significantly in the group Cd (CO: 0.346±0.02; Cd: 0.613±0.024; p: 0.0001); the expression of iNOS was representative in the group Cd (CO: 1.106±0.087; Cd: 1.284±0.08; p: 0.0244) but the levels of NO were significantly lower in this (CO: 1.568±0.053; Cd: 0.758±0.022; p: 0.0001). We concluded that Cd produces inflammation in cerebral cortex, as well as oxidative stress, induces the expression of iNOS but reduces the levels of NO due to the strong oxidizing environment in the cell interior.