Efecto de una dieta hipercalórica en pulmón de ratas hipoandróginas

ABALLAY FEDERICO; ZELARAYAN DANIELA; SALINAS M; SALINAS ELOY; RAZZETTO G; PEREZ CHACA MV; CIMINARI E; BIAGGIO VS; GOMEZ NN

San Luis

Congreso; XXXIX Sociedad de Biología de Cuyo; 2021

XXXIX Sociedad de Biología de Cuyo

Obesity is a systemic state of inflammation and oxidative stress thataffects normal respiratory physiology. The mediators and mediators behind theseprocesses are not well known yet. Even though obesity occurs more frequently inwomen, its effects on health are worse on men that on women. The interest onthe role of sex hormones as lung function modulators has risen in the lastyears. The presence of androgen receptors in lung reveal that these hormonesmight play a key role in lung physiology. Obesity-mediated oxidative stressproduced in adipose tissue is one of the main factors considered as oxidantsource and inflammation mediator. The aim of this work was to study the effectof diet-induced obesity on castrated rat lung. Wistar male rats (200 ± 20 g)were separated in four groups: Control with normal diet(CoN), castrated withnormal diet (KN), control with hypercaloric diet (CoOB) and castrated withhypercaloric diet (KOB) and sacrificed 30 days after castration. We analyzeddifferent parameters catalase activity (CAT), lipid peroxidation (TBARS),expression of Catalase (CAT) andAndrogen receptor (RA) anddetermination of triglycerides (TG),cholesterol (CL), high-densitylipoproteins (HDL), urea and lactate deshidrogenase (LDH) in serum. ANOVA andTukey test were used for statistical analyses. The results showed TBARS levelsincreased in KOB group compared to CoN p<(0.001) and KN p<(0, 01) groups,respectively. CAT activity wasincreased in KN (p<0.05) group, compared to control. HDL levels increased inCoOB (p<0.001), KN (p<0.001) and KOB (p<0.01) groups. Both, urea and TG determinations were increased in KN (p<0.001);p<0.01 and KOB (p<0.001) groups. CL was increased in CoOB (p<0.001);KOB (p<0.01) compared to CoN group and increased in KN (p<0.001) and KOB(p<0.001) compared to CoOB group. LDHlevels were increased in KN (p<0.05) group, while uric acid determinationnot shown significantly differences. CATexpression decreased in CoOB and KN groups, compared to control group.  RA expression increased in KN group comparedto control group. We previously demonstrated an important oxidativestress state in a castrated animal model. The obesity and castration could bealtering several biochemical parameters and probably inducing an inflammatorystate.