TNF receptor p55 signaling modulates IL-12/23p40 production in Yersinia enterocolitica-induced reactive arthritis

MAYORDOMO ANDREA CONSTANZA; JERÉZ MARÍA BELÉN; ARIAS JOSÉ LUIS; SILVA JUAN EDUARDO; CARGNELUTTI ETHELINA; GORLINO CAROLINA VIRGINIA; DI GENARO MARIA SILVIA

Ciudad Autónoma de Buenos Aires

Congreso; 1st LASID-SAI-FAIC meeting; 2015

The purpose of this work was to gain insight into a regulatory role of TNFRp55 signaling on IL12/23p40 production in Ye induced ReA. Therefore, we analyzed IL12/23p40 together with TNF, IL10,IFNg and IL17 after LPS stimulation of splenocytes of wildtype (WT) and TNFRp55/ mice.Splenocytes were used to evaluate in the same cell culture the cytokines of the innate and adaptive immune responses. To examine a temporal effect, the cytokines were measured at different time after LPS stimulation. The responseswere compared in splenocytes of naïve mice, and in these cells obtained from mice at day 14 after Ye infection, time that corresponded with arthritis onset in TNFRp55/mice.In addition, the effect of TNF on IL12p40 production by LPSstimulated macrophages of RAW 264.7 cell line was also evaluated.