Dendritic cells play a central role in the pathogenesis of Yersinia enterocolitica-induced reactive arthritis in TNFRp55 deficient mice

MAYORDOMO ANDREA CONSTANZA; SILVA JUAN EDUARDO; GORLINO CAROLINA VIRGINIA; ARIAS JOSÉ LUIS; BERON WALTER; DI GENARO MARIA SILVIA

Ciuidad Autonoma de Buenos Aires

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2017

Dendritic cells (DC) orchestrate immune responses presenting antigens to T lymphocytes and driving their activation and differentiation to effector T cells. In previous studies, we demonstrated that TNFRp55-deficient (TNFRp55-/-) mice develop reactive arthritis (ReA) after orogastric infection with Yersinia enterocolitica (Ye). The purpose of the present work was to study the contribution of DC in the pathogenesis of Ye-induced ReA in this mouse model. The DC were purified after their in vivo expansion by injection of Fms-like tyrosine kinase 3-Ligand (Flt3L)-transfected BL16 melanoma. Isolated WT and TNFRp55-/- DC were stimulated with lipopolysaccharide (LPS), and IL-12/23p40 levels were assessed in culture supernatants by ELISA. Moreover, these cells were in vitro infected with Ye, and then co-cultured with purified CD4+ T cells obtained from spleen of WT and TNFRp55-/- mice 5 days post-infection with Ye. After 5 days, T cell differentiation to Th1 and Th17 profiles were analyzed by flow cytometry, and IFN-and IL-17 levels were measured in culture supernatants. We found that TNFRp55-/- DC secreted higher levels of IL-12/23p40 compared with WT DCs (p