DETECTION OF INTESTINAL MICROBIOTA COMPONENTS IN THE SYNOVIAL MICROENVIRONMENT OF PERIPHERAL SPONDYLOARHRITIS AND ITS ASSOCIATION TO IMMUNOPATHOGENIC MECHANISMS

JOSE LUIS ARIAS; EDUARDO CALLEGARI; DANIELA PAEZ; BLAS RODRIGO; ALICIA MUNARRIZ; RODOLFO PARDO-HIDALGO; HECTOR TAMASHIRO; MARIA SILVIA DI GENARO

Evento virtual

Congreso; REUNION DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

Sociedades científicas SAIC, SAI, AAFE, NANOMED-AR

A role for intestinal microbes in arthritis is being considered. We analyzed intra-articular microbiota components and their association with immunopathogenic mechanisms of Spondyloarthritis (SpA). Synovial fluid (SF) of peripheral SpA patients were pooled (n=9): 6 psoriatic arthritis, 2 reactive arthritis, 1 undifferentiated SpA (Protocol approved CE002-2017). IL-17, IL-6, IL-23 and TGF-b levels were quantified in each SF sample by ELISA and compared with SF from osteoarthritis (OA). Moreover, SW982 cells (human synovial fibroblasts) were incubated with the SpA SF pool in absence or presence of polymyxin B (LPS inhibitor) and 48 h later the IL-6 levels in the supernatant were measured by ELISA. Furthermore, intestinal microbiota proteins were analyzed in the SpA SF pool through a Gel-LC bottom-up mass spectrometry-based proteomic study. Higher levels of IL-17, IL-6, IL-23 and TGF-b were detected in SpA compared with OA SF (p