Inhibition of naphthoquinones on biofilm formation of a foodborne pathogenic strain by oxidative stress

DI MARCO, NATALIA; PAEZ, PAULINA; GARCIA, CELINA; LUCERO ESTRADA CECILIA STELLA MARYS; CARLOS RODOLFO PUNGITORE

La Laguna, Tenerife

Congreso; COST action CM 1407. Final Meeting. Challenging organic syntheses inspired by nature from natural products chemistry to drug discovery; 2018

European Cooperation in Science and Technology

Naphthoquinones are widely distributed in nature and they have been used in phytomedicine because their wide variety of pharmacological properties1. In previous studies in our laboratory, we found that a group of new naphthoquinones, obtained by chemical semi-synthesis, inhibited biofilm formation of pork sausage and Yersinia enterocolitica strain was isolated by more than 80%. It is known that bacteria have a balance between reactive oxygen byproducts, named as reactive oxygen (ROS) and nitrogen species (RNS), and antioxidant defenses (AXO). When this balance is altered in favor of oxidants, oxidative stress is generated2, which causes irreparable cellular damage. The aim of this work was evaluate the relationship between the mechanism of action of 4 inhibitors naphthoquinones (Figure 1) of Yersinia enterocolitica bioserotype 1A / O: 7,8-8-8,19 (CLC001) biofilm formation with oxidative stress production. The generation of ROS was quantified by spectrofluorometry using the fluorescent probe H2-DCFDA (6-carboxy-2 ', 7'-dichlorodihydrofluorescein diacetate diacetoxymethyl ester) and the generation of RNS was quantified by Griess reaction. To determine AXO, the enzyme superoxide dismutase (SOD) activity was quantified by methionine, riboflavin, EDTA and NBT assay. In this communication, the results of this study will be discussed.