Detection of Yersinia sp in meat foods by nested-PCR and culture methods

LUCERO ESTRADA CECILIA STELLA MARYS; DI GENARO SILVIA; VELÁZQUEZ LIDIA DEL CARMEN; STEFANINI DE GUZMÁN ANA MARÍA

San Luis

Jornada; XXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo. IV Reunión Científica de la Sociedad Argentina de Microscopía (SAMIC).; 2006

Sociedad de Biología de Cuyo

Yersiniosis is a human disease widely distributed, caused by Yersinia enterocolitica. The present of Y. enterocolitica in chicken and porcine products at retail level in San Luis city, was investigated. The samples studied were: 17 pure pork sausages, 22 pork and beef sausages, 21 minced meat, 24 chicken carcasses and 9 chicken sausages. The samples were tested by nested PCR which target gene was yadA and by culture methods: 25g of the sample were homogenized during 90 s with 225 ml tripticase soy broth (TSB) and then incubated 18 h 25º C. After that, two aliquots were analyzed: 1 ml was studied by PCR and 100 ul were transferred to 10 ml of Modified Rappaport broth (MRB), and incubated at 4° C during 21 days.After the enrichment plates isolations were carried out on cefsulodin-irgasan-novobiocin agar (CIN) and Mac Conkey agar (MC). The cultures were incubated during 48 h at 25° C. Four strains were isolated and bioserotyped as Y. enterocolitica B:2 O:9, Y. enterocolitica B1 A, Y. intermedia B:2 auto agglutinable (AA), and Y. intermedia B:2 AA. By nested PCR, the yadA positive samples were: 7 samples (41.17%) from pure pork sausage, 7 samples (31.81%) from beef and pork sausage and 12 samples (57.14%) from minced meat. The strain Y. enterocolitica B:2 O:9 was detected by nested-PCR and culture methods too. The nested-PCR was more sensitive to detect Y. enterocolitica from different foods than the culture methods.