Influence of glucose concentrations in Yersinia enterocolitica biofilms

CECILIA S. M. LUCERO ESTRADA; SILVIA DI GENARO; ANA MARÍA STEFANINI DE GUZMÁN

La Punta, San Luis

Jornada; XXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2009

Sociedad de Biología de Cuyo

Yersinia enterocolitica (Ye) is an enteropathogen with a biphasic lifestyle alternating between the food/water environment and the mammalian gastrointestinal tract. Bacterial in biofilms are more resistant to antimicrobial agents than in planktonic form, and this feature is determined by many factors. Previously, we demonstrated the ability of Ye to form biofilms on abiotic surfaces. The aim of the present work was study the influence of different glucose concentrations, in the culture medium, on the ability to form biofilms on abiotic surfaces. Four Ye "nonvirulent" biotype 1A strains, and 4 Ye biotype 1B virulent strains were studied. The culture medium was trypticase soy broth (TSB) without and added with glucose in the following concentrations: 0.25, 0.5, 0.75, 1.00 and 2.00% (TSBG). After an overnight culture, 100 ul of each strain were added to 10 ml of TSBG, put into a 24-well microtiter plate, and cultured for 72 h at 25 or 37°C. Then, the ability to form biofilm was measured by staining with 1% crystal violet (CV), solubilizing with 96° ethanol, and finally, reading the optica] density at 590 nm (D0590nm).The glucose concentrations that enhanced the Ye biofilm formation were 0.50 and 0.75% at 48 h culture at both temperatures (p