DNA restriction profiles of Salmonella Enteritidis strains isolated from chicken in San Luis, Argentina.

FAVIER GABRIELA ISABEL; LUCERO ESTRADA CECILIA STELLA MARYS; SORIA JOSÉ; LAZARTE OTERO VALERIA; ESCUDERO MARÍA ESTHER; VELÁZQUEZ LIDIA DEL CARMEN

Mendoza

Jornada; XXVIII Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2010

Sociedad de Biología de Cuyo

Pulsed field gel electrophoresis (PFGE) is a powerful tool applied to investigate the heterogeneity of the genomic restriction profiles of bacteria belonging to the same species and establish relations of epidemiological relevance. In the present work, this technique was used for subtyping of Salmonella Enteritidis strains isolated from 113 chicken intended for consumption in San Luis, Argentina. The preparation of chromosomal DNA of each strain in agarose plugs, digestion with XbaI, and electrophoresis of the resulting macrorestriction fragments were performed according to the PFGE protocol standardized by PulseNet (Centers for Disease Control and Prevention, CDC, USA). Electrophoresis was carried out using a CHEF-DR III system (BioRad) at 6 V/cm at 14°C for 20 h with the following pulse time: initial time 2.2 s and final time 63.8 s. The gel was stained with Gel Red (Biotium) and observed with a UV transilluminator. Salmonella Braenderup H9812 were used as DNA size standards. Identical DNA restriction patterns were observed for seven local S. Enteritidis strains. The similarity of DNA profiles of these strains suggested a common contamination source which might be located in the farm, the slaughterhouse or the retail store.