MINIMUN IHIBITORY CONCENTRATION (MIC) OF ANTI-Yersinia enterocolitica BIOFILMS ALKALOIDS.

DI MARCO, NATALIA; LUCERO ESTRADA CECILIA STELLA MARYS; CARLOS RODOLFO PUNGITORE

Potrero de los Funes, San Luis

Jornada; XXX Reunión Científica Anual Sociedad de Biología de Cuyo.; 2012

Sociedad de Biología de Cuyo

The biofilm mode of growth results in an increased bacterial resistance against classical antimicrobial treatment. Thus, the search for alternative therapies is a necessity. Previously a screening was performed among 16 different alkaloids and four of them (compounds numbers 3, 6, 10 and 11) were selected for their ability to inhibit Yersinia enterocolitica (Ye) biofilm formation at a concentration of 500 uM. The aim of this study was to determinate the minimum inhibitory concentration (MIC) of these alkaloids against Ye B1A/O:7,8-8-1,9 (CLO001) strain. This strain was selected for its high ability to form biofilms onto polystyrene. The culture medium was trypticase soy broth (TSB) added with 0.25% glucose and the biofilm formation was determined by the crystal violet technique after 24 h of incubation at 25°C onto polystyrene-96 well plates. The initial concentration of each compound was 500 M and serial dilutions 1/2 were performed. Sessile cells were measure at 550 nm. With compounds 3 and 10 the MIC observed for was 250 M and with compounds 11 the MIC was 31.25 uM. On the other hand, the most effective compound was number 6 with a MIC of 3.9 uM. Therefore, these kinds of compounds (primary number 6) could be use as new strategies to control microorganisms, specially the resistant biofilms.