INVESTIGADORES
LUCERO ESTRADA Cecilia Stella Marys
artículos
Título:
Depletion of dendritic cells enhances innate anti-bacterial host defense through modulation of phagocyte homeostasis
Autor/es:
STELLA E. AUTENRIETH; PHILIPP WARNKE; GUIDO H. WABNITZ; CECILIA LUCERO ESTRADA; KARINA A. PASQUEVICH; DOREEN DRECHSLER; MANINA GUNTER; KRISTIN HOCHWELLER; ANA NOVAKOVIC; SANDRA BEER- HAMMER; YVONNE SAMSTAG; GÜNTER J. HÄMMERLING; NATALIO GARBI; INGO B. AUTENRIETH
Revista:
PLOS PATHOGENS
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2012 p. 1 - 16
ISSN:
1553-7366
Resumen:
Dendritic cells (DCs) as professional antigen-presenting cells play an important role in the initiation and modulation of the adaptive immune response. However, their role in the innate immune response against bacterial infections is not completely defined. Here we have analyzed the role of DCs and their impact on the innate anti-bacterial host defense in an experimental infection model of Yersinia enterocolitica (Ye). We used CD11c-diphtheria toxin (DT) mice to deplete DCs prior to severe infection with Ye. DC depletion significantly increased the survival after Ye infection. The bacterial load in the spleen of DC-depleted mice was significantly lower than that of the control mice throughout the infection. DC depletion was accompanied by an increase in the serum chemokine levels of CXCL1, G-CSF, IL-1α, and CCL2 and an increase in the numbers of splenic phagocytes. Functionally, splenocytes from DC-depleted mice exhibited an increased bacterial killing capacity compared to splenocytes from control mice. Cellular studies further showed that this was due to an increased production of reactive oxygen species (ROS) by neutrophils. Adoptive transfer of neutrophils from DC-depleted mice into control mice prior to Ye infection reduced the bacterial load to the level of Ye-infected DC-depleted mice, suggesting that the increased number of phagocytes with added ROS production account for the decreased bacterial load. In summary, we could show that DC depletion triggers phagocyte accumulation in the spleen and enhances their anti-bacterial killing capacity upon Ye infection.
