Protective immune response induced by Yersinia enterocolitica deficient in YopH

DAVE MN; SILVA JE; GORLINO CV; DI GENARO MS

Mar del Plata

Congreso; LXII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2014

Sociedad Argentina de Inmunología

Yersinia outer protein H (YopH) is a powerful protein-tyrosin phosphatase that contributes to Yersinia enterocolitica (Ye) evasion. Previously, it has been demonstrated that Ye deficient in YopH (Ye DyopH) is attenuated in mice. However, the immune response induced by this mutant is unknown. The objective was to demonstrate whether previous Ye DyopH infection trigger a protective immune response against a consequent Ye wild-type or Salmonella enterica (Se) infection. For this purpose, C57BL/6 wild-type mice were orally infected with 1-5x108 Ye ΔyopH or they received saline (control). At day 14, the mice were challenged with Ye WA-314 (Ye wild-type) or Se. Survival, weight loss, clinical score and the colony forming units (CFU) number in Peyer´s patches (PP), mesenteric lymph nodes (MLN) and spleen at day 7 after challenger were determined. Cellular recruitment in PP at day 3 after Ye DyopH or Ye WA-314 infection were analysed by flow cytometry. We observed that Ye DyopHinfection induced significant increase in the survival (100% vs 60%, p