CpG-ODN induces, in vitro and in vivo, myeloid GR1+CD11b+ cells and suppressor functions in young and aged mice

HARMAN, M.F.; RANOCCHIA, R.; GORLINO, C.; MORÓN, G.; MALETTO, B.; PISTORESI, M.C.

Buenos Aires

Congreso; First French-Argentine Immunology Congress y LVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2010

SAI

Besides the ability of CpG-ODN to induce in aged (18 months: 18m) and young (3 months: 3m) BALB/c mice a powerful Th1 immune response, we showed that in vitro CpG-ODN is able to stimulate both, arginase and inducible nitric oxide synthase (iNOS) in macrophages. In the present study, we investigate the regulatory ability of CpG-ODN in vitro and in vivo in 3m and 18m mice and focused on the role of L-arginine metabolism. Bone marrow (BM) cells from 3m and 18m mice were incubated with GM-CSF (3?4 days), resulting in similar percentage of GR1+CD11b+ myeloid cells (3m: 50±4%, 18m: 55±5%). The treatment of BM-derived myeloid cells with CpG-ODN plus IFN-gamma induces arginase activity and nitric oxide (NO) production in both mice ages (p