Neutrophil migration to lymph nodes through lymph and blood in an immune inflammation model

GORLINO, C.; RANOCCHIA, R.; MORÓN, G; MALETTO, B.; PISTORESI, M.C.

Viña del Mar

Congreso; 9th Latin American Congress of Immunology; 2009

ALAI

We previously showed that when OVA-FITC was injected into the footpad of OVA/CFA immunized mice, the main OVA-FITC+ cells recruited in draining popliteal lymph nodes were neutrophils and this influx was dependent on an antigen-specific response. On the basis of these findings, we investigated if neutrophils can enter into lymph nodes trough afferent lymphatics and blood. Immunofluorescence of lymph node sections of OVA/CFA-immunized mice injected with OVA-FITC showed the presence of neutrophils OVA-FITC+ in the lumen of lymphatic vessels (LYVE-1+) and in the high endothelial venules (MECA-79+). Bone marrow neutrophils incubated with or without immune complexes (anti-OVA rabbit sera plus OVA) were labeled with CFSE and injected in the footpad or vein of OVA/CFA-immunized mice. Neutrophils were only found in draining popliteal lymph nodes of mice injected with neutrophils incubated with immune complexes (p