Searching for improved mimetic peptides inhibitors preventing conformational transition of amyloid-β 42 monomer

GERA, JÁNOS; SZÖGI, TITANILLA; BOZSÓ, ZSOLT; FÜLÖP, LIVIA; BARRERA, EXEQUIEL E.; RODRIGUEZ, ANA M.; MÉNDEZ, LUCIANA; DELPICCOLO, CARINA M.L.; MATA, ERNESTO G.; CIOFFI, FEDERICA; BROERSEN, KERENSA; PARAGI, GABOR; ENRIZ, RICARDO D.

BIOORGANIC CHEMISTRY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2018 vol. 81 p. 211 - 221

0045-2068

A series of novel mimetic peptides were designed, synthesised and biologically evaluated as inhibitors of Aβ 42 aggregation. One of the synthesised peptidic compounds, termed compound 7 modulated Aβ 42 aggregation as demonstrated by thioflavin T fluorescence, acting also as an inhibitor of the cytotoxicity exerted by Aβ 42 aggregates. The early stage interaction between compound 7 and the Aβ 42 monomer was investigated by replica exchange molecular dynamics (REMD) simulations and docking studies. Our theoretical results revealed that compound 7 can elongate the helical conformation state of an early stage Aβ 42 monomer and it helps preventing the formation of β-sheet structures by interacting with key residues in the central hydrophobic cluster (CHC). This strategy where early ?on-pathway? events are monitored by small molecules will help the development of new therapeutic strategies for Alzheimer´s disease.