DIET-INDUCED OBESITY IN MALE MICE IS ASSOCIATED WITH METABOLIC AND HISTOLOGICAL DISORDERS IN TESTIS

FUNES,A; ?SIMÓN L,; GOMEZ-MEJIBA S.E; SÁEZ LANCELLOTTI E; RAMIREZ D.C,; MIGUEL W. FORNES; DELLA VEDOVA MC

MZA

Congreso; SBC; 2014

Overweigh and obesity are known causes of infertility in men, but the mechanism involved remains unknown.The aim of the present study was to investigate both reproductive and metabolic features of C57BL/6 mice fed anormal chow (LFD, low fat diet) or a 60% HFD (high-fat diet). At the end of the experiment (18 weeks), bothreproductive and metabolic characteristics were assessed. Our data show that HFD caused significant metabolicalterations in mice, including obesity, glucose intolerance, dyslipidemia, and subtle liver steatosis. These findingssuggest that HFD induces metabolic features in mice. Moreover, HFD showed differences in the expression ofRNA messenger encoding proteins related to the synthesis and metabolism of cholesterol (SREBP-1, SREBP-2and HMGCoAreductase). Few changes were observed histologically, but marked variations at the molecular levelby comparing mice with control diet and fat. These data suggest that the abnormalities foundin spermatogenesismay be caused by changes in metabolic status and the molecular machinery of testis.