Molecular typing of Mycobacterium avium subsp. paratuberculosis in dairy cattle herds of Antioquia, Colombia

CORREA-VALENCIA NATHALIA; FERNANDEZ-SILVA JORGE; MOYANO ROBERTO DAMIAN; ROMANO, MARÍA ISABEL

REVISTA MVZ CORDOBA

UNIV CORDOBA

Año: 2020 vol. 25

0122-0268

To determine Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in invironmental samples from Colombian dairy herds. Materials and methods. Environmental samples from 25 IS900-qPCR MAP-positive dairy herds were cultured by duplicate in Herrold´s egg yolk medium with mycobactin J to obtain isolates. Suspicious colonies were confirmed by MAPIS900-qPCR. Positive DNA was sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) and multilocus short sequence repeats (MLSSR) techniques to analyze the genetic differences between the isolates. Results. Sub-typing revealed two different genotypes by MIRU-VNTR (INMV 2 and INMV 36). MLSSR technique was carried out to increase the discriminatory power from what was obtained by MIRU-VNTR, but no differences were observed among the recovered isolates. Conclusions. The present study represents an important approach to the knowledge on MAP epidemiological status in the study population.