DENDRITIC CELLS ARE RESPONSIBLE FOR THE FAILURE IN CTLs GENERATION IN LSP1-DEFICIENT MICE

GROS, MARINE; PASCUAL, MARÍA MERCEDES; MORON, VICTOR GABRIEL; ACLAND STRACK, RACHEL PAOLA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Inmunología

Leukocyte-specific protein 1 (LSP1) is an important regulator of actin cytoskeleton remodeling, modulating leukocytes motility, due to its F-actin binding domain. We have previously shown that Lsp1- /- mice have an impaired CTL response after antigen exposure. Moreover, Lsp1-/- dendritic cells (DCs) fail to induce a strong CTL response in vivo, migrate to lymphoid tissues, present antigens and produce IL-12 when transferred into WT mice.In order to deepen the mechanisms operating this diminished CTL generation in Lsp1-/- mice, we first analyzed the ability of their CD8+ T cells to proliferate and become activated. After in vitro stimulation with αCD3/CD28, Lsp1-/- CD8+ T cells proliferated and up-regulated CD25 as strongly as WT CD8+ T cells. Granzyme B production and IFNg release were slightly lower (p