EFFECT OF CIRCADIAN VARIATIONS IN MUCOSAL IMMUNE RESPONSE TO FOOD ANTIGEN

FRANCO NICOLAS NAZAR; MACCIÓ MARETTO, LISA; CORREA SILVIA GRACIELA; BARRIOS BIBIANA ELISABET

Buenos Aires

Congreso; Reunión conjunta de sociedades de biociencia; 2017

Many immune parameters oscillate rhythmically with the time of day and the circadian clock has emerged as an important gatekeeper that enhances fitness of the organism. In the present work we have characterized the influence of biological rhythms on the immune function of the intestinal mucosa and its impact on the tolerance towards oral antigens. Using multivariate statistic tests (PCA , ADL, MANOVA), we evaluated 17 parameters (cell subsets, homing receptors, activation markers and cytokines) in mesenteric lymph nodes (MLN) of B6 WT mice exposed to light-dark 12:12 conditions at four times (ZT5, 11, 17 and 23). PCA and ADL tests confirmed that over a 24-hr period, three different immune configurations can be described in MLN: at the light period (ZT 5); at the dark period (ZT17) and at the transition periods (ZT11 and 23). The distribution in these groups was dependent on the integrity of the molecular clock, as mice deficient in the clock gene Per2 (Per2KO), showed significant differences with WT animals (p