Effects of 5-Fluorouracil on myeloid derived supresor cells subsets and its consequence in the CD62L expression on CD4 and CD8 T cells during Trypanosoma cruzi infection

AROCENA AR; ONOFRIO LI; PAROLI A; GEA S

Los Cocos

Congreso; LXI Reunion Anual de la SAI; 2013

Myeloid derived suppressor cells (MDSC), CD11b+Gr1+, are a heterogeneous cell population consisting of immature cells. They are considered to be one of the major components of the immune suppressive network that suppress T cell responses in pathological conditions. Previously we described an increase of MDSC with an important rol in controlling the pro-inflammatory immune response during T. cruzi acute infection. We study the relevance of MDSC in this parasitic infection through the use of 5-fluorouracil(5FU), which selectively induces MDSC apoptotic cell death. We evaluated on infected BALB/c mice different consequences of the MDSC reduction. Infected BALB/c mice received a single i.p. injection of 5FU at 50 mg/kg. Mice injected with PBS were used as untreated controls. Results: there was a reduction on CD4 and CD8 T cells of CD62L levels from infected mice in comparison with uninfected ones (CD4: 41,3±1,2 vs 63±0,7% -p