THE CHEMOKINE RECEPTOR CXCR4 MEDIATES NEUTROPHIL MIGRATION TO LYMPH NODES FROM BOTH BLOOD AND LYMPHATIC ROUTES

GORLINO, C; CASTELL,S; HARMAN,MF; MORÓN, G; MALETTO, B; PISTORESI, MC

Los Cocos. Cordoba

Congreso; LXI Reunión de la Sociedad Argentina de Inmunología.; 2013

Sociedad Argentina de Inmunologia

Although much is described about the molecules involved in neutrophil migration from the circulation into tissues, less is known about the molecular mechanisms that regulate neutrophil entry into lymph nodes draining a local inflammatory site (dLN). The aim of this study was to determine if chemokine receptors are involved in neutrophil migration to dLN in a context of inflammation induced by immunization of BALB/c mice with ovalbumin (OVA) emulsified in Complete Freund?s Adjuvant (CFA). By confocal and flow cytometry analysis, we showed that neutrophils can enter dLN of OVA/CFA mice via lymphatic vessels and also from blood, across high endothelial venules. Additionally, we found that neutrophil migration to dLN could be abrogated by pertussis toxin treatment (p