ABSENCE OF CD39 MAY INDUCE A PRE-EXHAUSTED PHENOTYPE ON TUMOR-INFILTRATING CD8+ T LYMPHOCYTES

ABRATE, CAROLINA; RODRIGUEZ, CONSTANZA; MONTES CAROLINA L; BOSSIO, SABRINA N.; GRUPPI, ADRIANA; BOCCARDO, SANTIAGO; ACOSTA RODRIGUEZ E V

Congreso; Reunion anual SAI, SAIC, AAFE, NANOMED-AR; 2021

SAI, SAIC, AAFE, NANOMED-AR

ABSENCE OF CD39 MAY INDUCE A PRE-EXHAUSTED PHENOTYPE ON TUMOR-INFILTRATING CD8+ T LYMPHOCYTESAbrate Carolina1, Bossio Sabrina1, Boccardo Santiago1, Rodriguez Constanza1, Gruppi Adriana1, Acosta Rodriguez Eva1, Montes Carolina1. 1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI-CONICET), Haya de la Torre y Medina Allende, Ciudad Universitaria, Córdoba, Argentina.Revista Medicina Vol 81 Supl. III-2021. Reunion de Sociedades de Biociencias SAIC-SAI-AAFE-NANOMED-ARWe previously demonstrated that CD39KO tumor-bearing mice exhibit a higher % of tumor-infiltrating (TI) CD8+ T cells with effector phenotype as well as a higher % of specific TI-CD8+ T cells than Wild type (WT). CD39 participates in the generation of adenosine, an immunosuppressive molecule that interferes in the anti-tumor response. In this work, we evaluated the role of CD39 on TI-CD8+ T cells. C57BL/6 WT and CD39KO mice were injected with 1x106 B16F10-OVA tumor cells, 17 days after injection, we evaluated by flow cytometry the expression of inhibitor receptors (IRc) (PD-1 and TIM-3), transcription factors (TF) related to exhaustion (TOX, TCF-1, EOMES, T-bet and IRF-4) and cytotoxic related molecules (granzime B (GzB), perforin, and CD107) on TI-CD8+ T cells. CD39 KO and WT tumor bearing mice exhibited two PD-1 expressing populations: a PD-1high and PD-1low; however, CD39KO mice exhibited a higher % of PD-1low TI-CD8+ T cells than WT(p