IBODA 05360
INSTITUTO DE BOTANICA DARWINION
Unidad Ejecutora - UE
artículos
Título:
Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Autor/es:
NELSON ROMANO; ANDREA GIOFFRÉ; SILVANA M. SEDE; ELEONORA CAMPOS; ANGEL CATALDI; PAOLA TALIA
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Nueva York; Año: 2013 p. 1 - 10
ISSN:
0343-8651
Resumen:
Cellulolytic activities of three bacterial consortiaderived from a forest soil sample from Chaco region,Argentina, were characterized. The phylogenetic analysis ofconsortia revealed two main highly supported groupsincluding Achromobacter and Pseudomonas genera. Allthree consortia presented cellulolytic activity. The carboxymethylcellulase(CMCase) and total cellulase activitieswere studied both quantitatively and qualitatively andoptimal enzymatic conditions were characterized andcompared among the three consortia. Thermal and pH stabilitywere analyzed. Based on its cellulolytic activity, oneconsortium was selected for further characterization byzymography. We detected a specific protein of 55 kDa withCMCase activity. In this study, we have shown that theseconsortia encode for cellulolytic enzymes. These enzymescould be useful for lignocellulosic biomass degradation intosimple components and for different industrial applications.
