MOUSE PATERNAL ALCOHOL EXPOSURE AFFECTS SPERMATOZOA AND COMPROMISES THE SURVIVAL OF ITS OFFSPRING

GOTFRYD L.; SALOMONE G.; CAMBIASSO M.; NAGUILA Z.; STINSON M.; ERLEJMAN A.G.; CALVO J.C.; FONTANA V.

Mar del Plata

Congreso; LXIII Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Sociedad Argentina de Investigación Clínica

Previously we observed that male alcohol consumption delayed embryodifferentiation by deregulation of peri-implantation events andalteration of embryo morphogenesis in vitro. Aim: To evaluate theeffect of paternal alcohol consumption on spermatozoa, embryo andlitter survival on a mouse model. Methods: CF-1 male mice wereexposed (treated group, T) or not (control group, C) to 15% (v/v)ethanol in drinking water ad libitum for 15 days. Spermatozoa fromcauda were obtained by swim-out to determine sperm concentration,motility, head decondensation (15, 30 and 60 min) and apoptosisby Annexin V-FITC on both groups. Control and treated maleswere mated with non-treated CF-1 females (1:1). Pregnant females(positive vaginal plug: day1) were sacrificed at day 2 of gestationto obtain 2-cell embryos which were cultured in vitro for 7 days andapoptosis was determined. Pregnancy outcomes were also evaluatedand litter mortality, weight and size registered. Results: Malealcohol consumption did not alter the number of mated female butsignificantly increased sperm head decondensation compared tocontrols (p<0.05). There were not differences in sperm concentrationand motility. Besides, we observed a higher number of oocytesat day 2 of gestation from females mated with treated males thancontrol ones (p<0.01). Apoptosis was detected in peri-implantationembryos at 7 days of culture in vitro from treated vs. control groups.However, no differences were observed when we evaluated apoptosison spermatozoa. After 21 days of pregnancy we registered bornmice from both groups and observed during the first week of age anincreased number of deaths from treated group (p<0.001) comparedto control group. We observed less activity and poor fur in thosepups from treated group vs. control males. Conclusion: Short-termpaternal alcohol consumption impairs sperm head decondensationaltering the embryo survival and pups behavior at an early age.