Performance of Bradyrhizobium and Bradyrhizobium-Azospirillum in alleviating the effects of water restrictive conditions during the early stages of Arachis hypogaea growth

CESARI A; PAULUCCI NS; LÓPEZ GOMEZ M; HIDALGO CASTELLANOS J; LLUCH PLÁ C; DARDANELLI M.S

JOURNAL OF PLANT GROWTH REGULATION

SPRINGER

Lugar: Berlin; Año: 2019

0721-7595

The objective of the work is to evaluate whether Bradyrhizobium sp. SEMIA6144 and Azospirillum brasilense Az39 can be used in inoculation practices in order to mitigate the negative effect of water restriction on growth and interaction with Arachis hypogaea. In this study, Nitrogenase activity was determined by measuring the H2 evolution in an open flow system and N and C concentration in plants were determined by Elemental Analyzer. Lipid peroxidation and polyamines (PA) levels were analyzed by HPLC. The results showed that the restrictive water condition (RWC) caused an 80% inhibition of the N fixation rate. Single and double inoculation favored peanut growth under RWC being better the single inoculation with SEMIA6144. Peanut plants have higher numbers of nodules in the roots when inoculated with SEMIA6144 in the absence of Az39, although it wasobserved that the inoculation with Az39 favored root development thus allowing the appearance of more infection sites in peanut roots. In double inoculation, the demand for N in the peanut was met with greater effectiveness. PA found in leaves were Putrescine,Spermidine and Spermine. The results show that SEMIA6144 inoculation reversed the negative effects of RWC to a greater extent on growth and nodulation peanut parameters. Simultaneous application of SEMIA6144 and Az39 improved early nodulation, efficiency in N fixation and total N, improving tolerance of A. hypogaea to RWC. Our findings provide new insights in the context of mixed inoculation and improvement of peanut production in limited water environment.