One-tube nested polymerase chain reaction for detection of Chlamydia trachomatis.

CRIBB PAMELA; SCAPINI JUAN PABLO; SERRA ESTEBAN

MEMORIAS DO INSTITUTO OSWALDO CRUZ

Instituto Oswaldo Cruz- Fiocruz- Rio de Janeiro- Brasil

Lugar: Rio de janeiro; Año: 2002 vol. 97 p. 897 - 900

Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.