PROTON CHANNEL INHIBITION ALTERS CELL PROLIFERATION AND CELL CYCLE IN BREAST CANCER CELLS

C VENTURA; A ASUAJE; N ENRIQUE; P MARTÍN; M NÚÑEZ; C COCCA; V MILESI

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Metabolic reprogramming of cancer cells conduces to a high production of acidic substances which must be extruded to maintain cell viability. In this work, we studied the effect of 2-(6-chloro-1H-benzimidazol-2-yl)guanidine (ClGBI), an inhibitor of the proton channel Hv1, on proliferation and cell cycle of tumorigenic (MDA-MB-231 and MCF-7) and non-tumorigenic (MCF-10A) human breast cell lines. Dose-response curve (0-20μM) and time-course (10-48h) were assayed to study cell proliferation (clonogenic assay), cell viability (MTT), cell cycle (flow cytometry) and mitotic cells (immunofluorescence using DAPI and anti-α-tubulin antibody). Results: ClGBI decreased the clonogenicity of the three cell lines in a concentration dependent way, getting the maximal inhibition at 10μM (p