Histamine regulates the MAPK pathway via the H2 receptor in PANC-1 human cells

G. CRICCO; G. MARTÍN; V. MEDINA; M. NÚÑEZ; A. GUTIÉRREZ; C. COCCA; R. BERGOC; E. RIVERA

Inflammation Research

Año: 2004 vol. 53 p. 65 - 66

The role of histamine (HA) in normal and neoplastic growth has been extensively investigated. We have previously demonstrated that the human pancreatic carcinoma cell line PANC-1 overexpresses H1 and H2 receptors (H1R, H2R). Stimulation of the H2R by high HA concentrations or by specific agonists increases intracellular cAMP levels and inhibits cell growth without inducing apoptosis but producing a partial activation of cell differentiation. On the otherhand, cells synthesize and release HA and low concentrations of HA increase cell proliferation suggesting that the H1R could be also involved in the regulation of cell growth [1, 2]. It has been reported that HA modulates the Mitogen Activated Protein Kinase (MAPK) pathway via H1R in normal and tumoral cells [3]. In this work we evaluated the possible regulation of expression of MAPK activity through H2R. We proposed that an interaction between the p38 and ERK pathways is involved in HA regulation of cell growth via H2R.