ULTRASTRUCTURAL STUDY OF THE SMALL INTESTINE

TANO DE LA HOZ, MARÍA FLORENCIA; FLAMINI, MIRTA ALICIA; DÍAZ, ALCIRA OFELIA

Zavalla

Congreso; XV Congreso y XXXIII Reunión Anual de la Sociedad de Biología de Rosario; 2013

Sociedad de Biología de Rosario

The objective of this study was to describe the ultrastructure of the small intestine of Lagostomus maximus by transmission electron microscope (TEM). Samples from duodenum, jejunum and ileum were fixed in 3% glutaraldehyde in cacodylate buffer 0.1 M (pH 7.3) and routinely processed for TEM. In the epithelium of the intestinal mucosa four mature cell types were identified: enterocytes, goblet cells, Paneth cells and enteroendocrine cells. Enterocytes showed uniform and regular microvilli and presented on their lateral surface junctional complexes and interdigitations. Goblet cells showed numerous mucous granules distending the apical region of the cell and other organelles distributed in the cytoplasm. Paneth cells were observed in the bottom of the crypts. They had a basally located nucleus and the cytoplasm of the perinuclear region contained the rough endoplasmic reticulum and Golgi apparatus. The granules lay in the supranuclear region. In the basal portion of the intestinal glands enteroendocrine cells with abundant secretory granules were detected. In the duodenal submucosa, Brunner?s glands with mucous cells were identified. These gland cells presented abundant electrolucid granules in their apical cytoplasm, numerous mitochondria, and an extensive rough endoplasmic reticulum. The existence of highly specialized cells in the small intestine of L. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. microscope (TEM). Samples from duodenum, jejunum and ileum were fixed in 3% glutaraldehyde in cacodylate buffer 0.1 M (pH 7.3) and routinely processed for TEM. In the epithelium of the intestinal mucosa four mature cell types were identified: enterocytes, goblet cells, Paneth cells and enteroendocrine cells. Enterocytes showed uniform and regular microvilli and presented on their lateral surface junctional complexes and interdigitations. Goblet cells showed numerous mucous granules distending the apical region of the cell and other organelles distributed in the cytoplasm. Paneth cells were observed in the bottom of the crypts. They had a basally located nucleus and the cytoplasm of the perinuclear region contained the rough endoplasmic reticulum and Golgi apparatus. The granules lay in the supranuclear region. In the basal portion of the intestinal glands enteroendocrine cells with abundant secretory granules were detected. In the duodenal submucosa, Brunner?s glands with mucous cells were identified. These gland cells presented abundant electrolucid granules in their apical cytoplasm, numerous mitochondria, and an extensive rough endoplasmic reticulum. The existence of highly specialized cells in the small intestine of L. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. Lagostomus maximus by transmission electron microscope (TEM). Samples from duodenum, jejunum and ileum were fixed in 3% glutaraldehyde in cacodylate buffer 0.1 M (pH 7.3) and routinely processed for TEM. In the epithelium of the intestinal mucosa four mature cell types were identified: enterocytes, goblet cells, Paneth cells and enteroendocrine cells. Enterocytes showed uniform and regular microvilli and presented on their lateral surface junctional complexes and interdigitations. Goblet cells showed numerous mucous granules distending the apical region of the cell and other organelles distributed in the cytoplasm. Paneth cells were observed in the bottom of the crypts. They had a basally located nucleus and the cytoplasm of the perinuclear region contained the rough endoplasmic reticulum and Golgi apparatus. The granules lay in the supranuclear region. In the basal portion of the intestinal glands enteroendocrine cells with abundant secretory granules were detected. In the duodenal submucosa, Brunner?s glands with mucous cells were identified. These gland cells presented abundant electrolucid granules in their apical cytoplasm, numerous mitochondria, and an extensive rough endoplasmic reticulum. The existence of highly specialized cells in the small intestine of L. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. L. maximus is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa. and immunological functions performed by the intestinal mucosa. is related to the multiple digestive, absorptive, endocrine and immunological functions performed by the intestinal mucosa.