DETERMINATION OF LEAD TRACES IN HONEY USING A FLUORIMETRIC METHOD

MARÍA C TALIO; MUÑOZ, VANESA; ACOSTA MARIANO; FERNANDEZ, L

FOOD CHEMISTRY

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2019

0308-8146

Heavy traces metals may be present in honey being their detection very important for the quality control and it also serves as an indicator of environmental pollution. A new methodology for lead traces determination has been developed based on the quenching effect of the metal on fluorescent emission of 8-hydroxyquinoline and o-phenanthroline at λem = 360 nm (λexc = 250 nm). Experimental variables that influence on fluorimetric sensitivity were optimized by uni-variation assays. The calibration graph using zeroth order regression was linear from 0.105 µg L-1 to 51.8 µg L-1, with correlation coefficient better than 0.998. Under the optimal conditions, the limits of detection and quantification were of 0.035 µg L−1 and 0.105µg L−1, respectively. The trueness of the methodology was assessed trough parallel samples analysis by ICP-MS. The proposed method was applied to the determination of lead trace amounts in honey from San Luis city (Argentina) with satisfactory results.