Metsulfuron-methyl determination in environmental samples by solid surface fluorescence

ALESSO, MAGDALENA; ALMEIDA, CÉSAR A.; TALIO, MARÍA C.; FERNÁNDEZ, LILIANA P.

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Año: 2018 vol. 139 p. 150 - 154

0026-265X

A new environmental friendly methodology for metsulfuron-methyl quantificationbased on the fluorescent signal enhancement of rhodamine B dye has been developed. Acationic surfactant (cetyltrimethylammonium bromide, CTAB) and an anionic one (sodiumdodecylsulfate) were employed to preconcentrate the herbicide using a coacervationphenomenon, in sodium borate buffer medium (pH 9.2). The coacervate phase wascollected on a nylon membrane (0.45 μm) and the solid surface fluorescence signal wasdetermined (λexc = 515 nm, λem = 565 nm). Experimental variables that influence onpreconcentration step and fluorimetric sensitivity have been studied and optimized usingresponse surface methodology. Under optimal working conditions, a LOD of 0.17 μg L-1and a LOQ 0.53 μg L-1 was obtained. The zeroth order regression calibration was linearfrom 0.53 to 5.00 μg L-1. The method showed adequate sensitivity and selectivity, and wasapplied to the determination of trace amounts of metsulfuron-methyl in environmentalwater samples. The proposed methodology implies an alternative to traditional techniquesfor metsulfuron-methyl monitoring using an accessible instrument in control laboratories,representing a contribution in the toxicological and environmental areas for the monitoringof MSM in environmental samples, in agreement with the Green Chemistry