SYNOVIAL MICROENVIRONMENT OF SPONDYLOARTHRITIS IMPACT ON FIBROBLAST-LIKE-SINOVIOCYTE: ROLE OF IL-6 AND S100A8 PROTEIN

DI GENARO, MARÍA SILVIA; ELIÇABE, RICARDO JAVIER; SILVA, JUAN EDUARDO; BLAS, RODRIGO; MUNARRIZ, ALICIA; PARDO-HIDALGO, RODOLFO; TAMASHIRO, HÉCTOR

San Miguel de Tucuman

Congreso; LXVII Reunión Anual de la sociedad Argentina de Inmunología - SAI 2019; 2019

Sociedad Argentina de Inmunología

Introduction. The molecular mechanisms driving the distinct characteristic of fibroblastlikesynoviocytes (FLS) in spondyloarthritis (SpA) remain unknown. We have reported thatsynovial fluid (SF) of SpA patients induces interleukin (IL)-6 secretion by FLS.Furthermore, a proteomic study of SF of SpA showed the presence of the alarminS100A8.The objective of the present work was to study the effect of the synovial S100A8on the IL-6 production by FLS. The expression of alpha-smooth-muscle-actin (a-SMA), amiofibroblast marker, was also studied.Methods. The SF of SpA patients were pooled (n=9). Human FLS of the SW982 cell linewere stimulated with the SF pool in absence or presence of anti-S100A8/A9 antibodies.Different concentration of the S100A8 (1-10 ug/ml) were also assayed. After 24-96 h, theIL-6 levels in the supernatants were measured by enzyme-linked immunosorbent assay(ELISA). Moreover, FLS were stained intracellularly for a-SMA and analyzed by flowcytometry. The data were analyzed by the two-way analysis of variance (ANOVA)followed by Bonferroni posttest. A p value < 0.05 was considered statistically significant.Results. We confirm that SpA SF induces IL-6 production by FLS (p