DENDRITIC CELLS PLAY A CENTRAL ROLE IN THE PATHOGENESIS OF Yersinia enterocolitica-INDUCED REACTIVE ARTHRITIS IN TNFRp55 DEFICIENT MICE

MAYORDOMO AC; SILVA, JE.; GORLINO CV; ARIAS JL; BERON W; DI GENARO MS

Buenos Aires

Congreso; Reunión Conjunta de sociedades de Biosciencias; 2017

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) ; SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) , SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); SOCIEDAD ARGENTINA DE INVESTIGACIÓN BIOQUÍMICA Y BIOLOGÍA MOLECULAR (SAIB); SOCIEDAD ARGENTINA DE FISIOL

DENDRITIC CELLS PLAY A CENTRAL ROLE IN THEPATHOGENESIS OF Yersinia enterocolitica-INDUCEDREACTIVE ARTHRITIS IN TNFRp55 DEFICIENT MICEAndrea Constanza Mayordomo (1), Juan Eduardo Silva (1),Carolina Virginia Gorlino (1), José Luis Arias (1), WalterBerón (2), María Silvia Di Genaro (1)(1) División de Inmunología, Facultad de Química, Bioquímicay Farmacia, Universidad Nacional de San Luis e InstitutoMultidisciplinario de Investigaciones Biológicas (IMIBIO),CONICET, San Luis, Argentina, (2) Instituto de Histologíay Embriología, Facultad de Ciencias Médicas, UniversidadNacional de Cuyo - CONICET, Mendoza, Argentina.Dendritic cells (DC) orchestrate immune responses presentingantigens to T lymphocytes and driving their activation and differentiationto effector T cells. In previous studies, we demonstratedthat TNFRp55-deficient (TNFRp55-/-) mice develop reactive arthritis(ReA) after orogastric infection with Yersinia enterocolitica (Ye). Thepurpose of the present work was to study the contribution of DC inthe pathogenesis of Ye-induced ReA in this mouse model. The DCwere purified after their in vivo expansion by injection of Fms-liketyrosine kinase 3-Ligand (Flt3L)-transfected BL16 melanoma. IsolatedWT and TNFRp55-/- DC were stimulated with lipopolysaccharide(LPS), and IL-12/23p40 levels were assessed in culture supernatantsby ELISA. Moreover, these cells were in vitro infected with Ye,and then co-cultured with purified CD4+ T cells obtained from spleenof WT and TNFRp55-/- mice 5 days post-infection with Ye. After 5days, T cell differentiation to Th1 and Th17 profiles were analyzed byflow cytometry, and IFN-g and IL-17 levels were measured in culturesupernatants. We found that TNFRp55-/- DC secreted higher levelsof IL-12/23p40 compared with WT DCs (p