EMBRYOTOXICITY OF CADMIUN IN ZEBRAFISH EGSS

PISTONE C; GIANNINI F; ENRIZ D; ALVAREZ SM; SANCHEZ ES

Congreso; 39na Reunión Anual de la Sociedad de Biologia de Cuyo; 2021

Fishes have been used in toxicology since 1950 showing formidable characteristics makingthem excellent for experimental models for studies in embryology and research in other areassuch as neurobiology, endocrinology and environmental biology. Their ability to adapt to a widevariety of environments, easy and short time of reproduction, rapid development, few nutritiondemands and low cost and easy maintenance in laboratory conditions among many otheradvantages provide unique research opportunities including environmental contamination andits ecological consequences. Cadmium (Cd), a toxic element and an important environmentalcontaminant, have shifted with the dramatic increase of Cd industrial and agrochemical usage inthe twentieth century. In this context, our lab has a large experience working with zebrafish(Danio rerio) that come with exceptional biological tools as a model system for vertebratedevelopment. Cd is known as a developmental toxic. The aim of this study was to evaluate Cdeffects in Danio rerio eggs. For this purpose, zebrafish embryos were incubated for 120 hours in3, 2, 1.5, 1, 0.5, 0.25 and 0.15 mg/lt of Cd chloride solution in a 24 chamber. We followed theprocesses to determine hatching rate, survival and teratogenicity of the embryos. Our resultsindicated that exposure to Cd profoundly alters the hatching process. The survival is affected bythe Cd solutions of 3 and 2 mg/l. In contrast, the other solutions did not significant affect thesurvival of any embryos. In addition, all tested concentration of the metal axenic has producedteratogenic malformation in all zebrafish embryos