INVESTIGADORES
RAMIREZ Dario
congresos y reuniones científicas
Título:
hypothyroidism, thyroid hormone receptors and inflammationin the heart
Autor/es:
CARMONA, Y.; SANDRA E. GOMEZ-MEJIBA; DARIO C. RAMIREZ; MARIA S. GIMENEZ
Lugar:
PUERTO MADRYN, CHUBUT
Reunión:
Congreso; SAIB 2010; 2010
Resumen:
CB-P06
HYPOTHYROIDISM, THYROID HORMONE RECEPTORS
AND INFLAMMATION IN THE HEART
Carmona Y , Gomez-Mejiba SE , Ramirez DC , Oliveros L ,
Gimenez MS
UNSL, IMIBIO-SL CONICET. Oklahoma Medical Research
Foundation, OK, USA. E-mail: yami.cv@unsl.edu.ar
The superfamily of nuclear receptors includes a number of
transcription factors, which activity regulates inflammation,
oxidative stress and metabolism. However, the effect of
hypothyroidism on heart inflammatory profile is unclear. Herein, we
sought to determine whether hypothyroidism affects nuclear
receptors expression and inflammation in the heart. To accomplish
this aim, a hypothyroidism model was produced in female virgin
Wistar rats (150-180 g body weight) by treating them with
(hypothyroid, HT) or without (euthyroid, ET) 100 mg/L of 6-npropyl
2-thiouracil in their drinking water. After 30 days of
treatment HT and ET rats were sacrificed and their heart excised for
the determination of PPARã, TNF-á, TRá, TRâ and â-actin (internal
control) mRNA by RT-PCR. The protein level of cyclooxygnease-2
(COX-2), inducible nitric oxide synthase (iNOS) and endothelial
NOS (eNOS) was determined by Western blot. Confocal imaging of
the heart tissue and RT-PCR data are consistent with a reduced
expression of TR-á1 and TR-â1, whereas increased COX-2 and
iNOS proteins, in the heart of HT with respect to ET rats. However,
we observed no changes in eNOS, PPAR-ã mRNA and TNF-á. Our
results suggest that hypothyroidism affects the inflammatory profile
in the heart. These results impact our understanding of
hypothyroidism-associated heart diseases.