INVESTIGADORES
LUCCHESI paula Maria Alejandra
artículos
Título:
Serovar distribution of a DNA sequence involved in the antigenic relationship between Leptospira and equine cornea
Autor/es:
LUCCHESI P.M.A.; PARMA A.E.; ARROYO, G.H.
Revista:
BMC MIcrobiology
Editorial:
BioMed Central
Referencias:
Año: 2002 vol. 2 p. 3 - 7
Resumen:
Background: Horses infected with Leptospira present several clinical disorders, one of them being
recurrent uveitis. A common endpoint of equine recurrent uveitis is blindness. Serovar pomona has
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
recurrent uveitis. A common endpoint of equine recurrent uveitis is blindness. Serovar pomona has
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
Horses infected with Leptospira present several clinical disorders, one of them being
recurrent uveitis. A common endpoint of equine recurrent uveitis is blindness. Serovar pomona has
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
pomona has
often been incriminated, although others have also been reported. An antigenic relationship
between this bacterium and equine cornea has been described in previous studies. A leptospiral
DNA fragment that encodes cross-reacting epitopes was previously cloned and expressed in
Escherichia coli.
Results: A region of that DNA fragment was subcloned and sequenced. Samples of leptospiral
DNA from several sources were analysed by PCR with two primer pairs designed to amplify that
region. Reference strains from serovars canicola, icterohaemorrhagiae, pomona, pyrogenes, wolffi,
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
DNA from several sources were analysed by PCR with two primer pairs designed to amplify that
region. Reference strains from serovars canicola, icterohaemorrhagiae, pomona, pyrogenes, wolffi,
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
A region of that DNA fragment was subcloned and sequenced. Samples of leptospiral
DNA from several sources were analysed by PCR with two primer pairs designed to amplify that
region. Reference strains from serovars canicola, icterohaemorrhagiae, pomona, pyrogenes, wolffi,
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
canicola, icterohaemorrhagiae, pomona, pyrogenes, wolffi,
bataviae, sentot, hebdomadis and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
and hardjo rendered products of the expected sizes with both pairs of
primers. The specific DNA region was also amplified from isolates from Argentina belonging to
serogroups Canicola and Pomona. Both L. biflexa serovar patoc and L. borgpetersenii serovar tarassoviL. biflexa serovar patoc and L. borgpetersenii serovar tarassovi
rendered a negative result.
Conclusions: The DNA sequence related to the antigen mimicry with equine cornea was not
exclusively found in serovar pomona as it was also detected in several strains of Leptospira belonging
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
exclusively found in serovar pomona as it was also detected in several strains of Leptospira belonging
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
The DNA sequence related to the antigen mimicry with equine cornea was not
exclusively found in serovar pomona as it was also detected in several strains of Leptospira belonging
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
pomona as it was also detected in several strains of Leptospira belonging
to different serovars. The results obtained with L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
L. biflexa serovar patoc strain Patoc I and L.
borgpetersenii serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
serovar tarassovi strain Perepelicin suggest that this sequence is not present in these
strains, which belong to different genomospecies than those which gave positive results. This is an
interesting finding since L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.
been associated clinically with equine uveitis.
L. biflexa comprises nonpathogenic strains and serovar tarassovi has not
been associated clinically with equine uveitis.