Identification of proteins involved in Hg-Se antagonism in water hyacinth (Eichhornia crassipes)

PABLO PACHECO; JULIO LANDERO; TRACI HANLEY

METALLOMICS

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2014

1756-5901

Different studies have established the presence of a proteinaceus complex involved in Hg/Se agonism/antagonism in plants. In order to identify proteins involved in this mechanism, water hyacinth plants were divided into groups and supplemented with a Hg, Se and Hg/Se mix. Proteins involved were identified through a screening separation with SEC-ICPMS followed by SAX-ICPMS and then peptide mapping of selected fractions with nanoLC-ESI-ITMS2. Total metal determinations showed that Se inhibits Hg translocation from roots to aerial compartments of the plant and that Se and Hg are antagonists to each other in terms of plant toxicity. In roots, stems and leaves Se was distributed mainly in two molecular mass fractions