A fully automated system for inorganic antimony preconcentration and speciation in urine

PABLO H. PACHECO, RAÚL A. GIL, LUIS D. MARTINEZ, GRISELDA POLLA, PATRICIA SMICHOWSKI

ANALYTICA CHIMICA ACTA

ELSEVIER SCIENCE BV

Año: 2007 p. 1 - 7

0003-2670

A studywas undertaken to ascertain the analytical capabilities of l-methionine immobilized on controlled pore glass for Sb preconcentration and speciation. A fully automated on-line system, implemented with hydride generation (HG) and inductively coupled plasma optical emission spectrometry (ICP OES), was used. Sb(III), at pH 10 was selectively retained in the column containing the immobilized aminoacid, while Sb(V) was not retained at all. A 30% HCl solution was used as eluent agent. Prior to total Sb determination, a pre-reduction step with thiourea was necessary. An on-line pH adjusting and pre-reduction of Sb(V) was achieved in a fully automated system. The detection limit for the preconcentration of 10mL of an aqueous solution was 70ng L-1 with a relative standard deviation of 2%. An enrichment factor of 20 was achieved when 10mL of sample was passed through the system, reaching a throughput of 23 samples per hour. The method was successfully applied to the determination of Sb(III) and total Sb in urine.l-methionine immobilized on controlled pore glass for Sb preconcentration and speciation. A fully automated on-line system, implemented with hydride generation (HG) and inductively coupled plasma optical emission spectrometry (ICP OES), was used. Sb(III), at pH 10 was selectively retained in the column containing the immobilized aminoacid, while Sb(V) was not retained at all. A 30% HCl solution was used as eluent agent. Prior to total Sb determination, a pre-reduction step with thiourea was necessary. An on-line pH adjusting and pre-reduction of Sb(V) was achieved in a fully automated system. The detection limit for the preconcentration of 10mL of an aqueous solution was 70ng L-1 with a relative standard deviation of 2%. An enrichment factor of 20 was achieved when 10mL of sample was passed through the system, reaching a throughput of 23 samples per hour. The method was successfully applied to the determination of Sb(III) and total Sb in urine.-1 with a relative standard deviation of 2%. An enrichment factor of 20 was achieved when 10mL of sample was passed through the system, reaching a throughput of 23 samples per hour. The method was successfully applied to the determination of Sb(III) and total Sb in urine.