MEIOSIS IN COLUMBIA LIVIA (BIRDS: COLUMBIFORMES)

OLEA GABRIELA; AGUIRRE MA. VICTORIA; STOYANOFF TANIA; TODARO SANTIAGO; LOMBARDO DANIEL

BUENOS AIRES

Congreso; Reunión conjunta de sociedades de biociencias; 2017

Sociedad Argentina de Biología

Some authors associate active caspase 3 with events of cell proliferation and differentiation. In the present work, to establish the role of this protein during the events of the development embryo in Columba livia, the immunohistochemical detection (IHQ) of active caspase 3 protein was carried out. For this reason, the expression of active caspase 3 was used in histological preparations of C. livia. Females corresponding to stages (E) 40 to 25 days after hatching (dpe) were tested using an active anti-caspase 3 antibodies to a concentration of 1:600 to 4C ON and developed according to the indirect "L-streptoavidin biotin" protocol. Expression of said protein was examined in females at quantitative level via Western Blot (WB). By IHQ said molecule was expressed in the left ovary from the E41. In contrast, oogonias dispersed in the right gonad, do not express active caspase 3, indicating that these, in the right gonad, do not enter into meiosis. The expression of caspase 3 active in oogonia in division allows inferring that this molecule would be involved during the gonadal ontogeny in the processes of cell division. The expression of caspase 3 active in oogony in division allows inferring that this molecule would be involved during the gonadal ontogenesis in the processes of cell division. This inference is confirmed by the previously presented results of the expression of GnRHR in ovogonies from E41, at which point the cells are in cell division. By WB, an increase in the expression of caspase 3 was detected from E41, indicating the onset of meiosis at this time. Taken together with the results of IHQ, these data indicate that active caspase 3 is expressed in the embryonic ovary from E41 and its expression is gradually increased, concomitantly to the increase of germ line cells entering meiosis. Therefore, active caspase 3 is not a key regulator of apoptosis in C. livia gonads since positive immunodetection is associated with germline cells in differentiation.