Effect of Aristolochia argentina on glutamic oxaloacetic transaminase in hepatotoxicity produced by paracetamol

MARIO FRANCO MOYANO

Modalidad Virtual

Congreso; XXXIXReunión Científica Anual Sociedad de Biología de Cuyo; 2021

Sociedad de Biología de Cuyo

Aristolochia argentina (Aristolochiaceae) is a rhizomatous plant popularly known as ?Charrúa?, ?Charruga?, ?Patito?, ?Buche de pavo?.The roots of this plant are used in popular medicine as: diuretic, antidiarrheic, astringent and emenagogue. In previous phytochemical tests,the presence of flavonoid compounds in its infusion was verified. The liver damage produced by paracetamol is a widely used model forthe study of hepatoprotective substances that, when metabolized, produce free radicals that cause liver damage. A. argentina showed a highantioxidant activity of 265.8 μmol of Fe (II)/g (FRAP). Aristolochia bracteolata Lam. and Aristolochia giberti possess antioxidant activitytoo. The aim of this research was to evaluate the effect of the root infusion of A. argentina on Aspartate Aminotransferase in the injuryproduced by paracetamol. The plant material was collected in the province of San Luis. Root infusion prepared according to the ArgentinePharmacopoeia VI edition in concentrations of 10% of A. argentina was used. Wistar rats of both sexes, 150-180 g, were used. Liver injurywas caused by oral administration of 640 mg/kg of paracetamol. The experimental animals (n:6-8/group) were administered via oral 10ml/kg/day (during 3 days) of physiological solution (group 1), physiological solution plus paracetamol one hour after the last dose (group2) and infusion of A. argentina (during 3 days) plus paracetamol one hour after the last dose (group 3). One day later, blood samples wereobtained for the biochemical determination of Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline-Phosphatase(ALP), γ-GT (gamma glutamyltransferase), total protein and bilirubin. Biochemical determinations were carried out with manual analysistechniques using Wiener lab reagents. Parametric ANOVA method was used. The toxic dose of paracetamol (640 mg/kg) produced liverdamage, as indicated by the significant increase in AST values compared to the control group (62.17±3.31 vs. 42.24±4.03, p