Quantitative single serum dilution liquid phase competitive blocking ELISA for the assessment of herd immunity and expectancy of protection against foot-and-mouth disease virus in vaccinated cattle

ROBIOLO, BLANCA; LA TORRE, JOSE; DUFFY, SERGIO; LEON, EMILIO; TORRES, ADRIANA; MATTION, NORA

JOURNAL OF VIROLOGICAL METHODS

Elsevier

Año: 2010 vol. 166 p. 21 - 27

0166-0934

A single serum-dilution liquid phase ELISA (slpELISA) was standardized to be used for serological evaluation of herd immunity against foot-and-mouth disease. The absorbance value at a dilution 1:64 of each serum sample was interpolated in a standard  curve by plotting the antibody titers of six control sera determined by end point dilution  liquid phase ELISA (lpELISA), against the absorbance values for the same control sera  at 1:64 dilutions. A straight line was obtained by linear regression analysis (r>0.90) in  the titer range of 1.40 to 2.40. The reliability of the antibody titers was confirmed by the simultaneous titration of 60 cattle sera by slpELISA and lpELISA, which showed an acceptable correlation (R2 >0.87) for viral strains A24/Cruzeiro, A/Argentina/01,  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39 2 >0.87) for viral strains A24/Cruzeiro, A/Argentina/01,  O1/Campos and C3/Indaial. Titers obtained by both methods were not significantly  different (p>0.05), thus confirming that slpELISA could be used successfully to replace  the conventional serial dilution ELISA for the assessment of protection status of cattle  in epidemiological studies. In addition, this quantitative slpELISA provides an  adequate method for monitoring the effectiveness of vaccination campaigns and is also suitable for use for the assessment of seroconversion of naive animals during early stages of infection. 39